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HansaBioMed/ExoTEST Ready to Use Kit for Tumor-derived Exosome enrichment and quantification from biological fluids (tra
产品编号：HBM-RTK-PTF/TC
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HansaBioMed/ExoTEST Ready to Use Kit for Tumor-derived Exosome enrichment and quantification from biological fluids (tra
商品介绍

ExoTESTisa sandwich enzyme-linkedimmunoassayforin-vitroexosomequantification in humanserum,plasmaandurine.

 

Application:

ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.

Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.

SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.

Relatedproducts :

Exosomestandards

RNAextractionkit

Immunocaptureandisolationtools

品牌介绍

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。



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产品分类
EXO-DNA-PS: Isolation of circulating and EV-associated DNA from plasma and serum Biochemicals Exo-Prep Cell proliferation and fractionation assays EXO-DNAc-PS: Isolation of circulating and EV-associated DNA from plasma and serum Lyophilized and purified exosomes from urine EXO-DNAc-UC: Isolation of circulating and EV-associated DNA from urine and cell culture me EXO-DNA-UC: Isolation of circulating and EV-associated DNA from urine and cell culture med Monoclonal AB EXO-DNA-UC: Isolation of circulating and EV-associated DNA from urine and cell culture med EXO-DNAc-UC: Isolation of circulating and EV-associated DNA from urine and cell culture me Exo-TEST: ready to use ELISA quantification kits RNA Basic kit Lyophilized and purified exosomes from plasma Oxidative stress Lyophilized and purified exosomes from serum Lyophilized and purified exosomes from saliva ELISA Immunoplates Lyophilized and purified exosomes from different cell lines PURE-EVs:Size exclusion chromatography columns TumorEXO-TotalRNA: Tumor Exosome Immunocapture and RNA extraction kit Lyophilized and purified exosomes from cell culture medium Fluorescent labeled Exosome Standards Immunobeads Polyclonal AB EXO-TotalRNA: Overall Exosome Immunocapture and RNA extraction kit
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Sampletype

Plasma,serum,urine.

Startingmaterial

Upto100ulofBIOLOGicalsamplecanbeloadedperwell.

Unfractionatedplasmasamplecanbedirectlyusedforcapture.

Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol.

Assaytype

Sandwichenzyme-linkedimmunoassay.

Sensitivity

Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein.

Productoverview

ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasma,serum,urineandcellculturesupernatant. 

ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit.

Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage.

ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies.

Primaryantibody

α-CD9murineantibody.

Exosomestandard

LyophilizedexosomesfromCOLO-1Cellculturesupernatant(seedetails).

Assayprotocol

Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection.

Assaycalibration:kitcontainsexosomestandardsforassaycalibration.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber.

Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples.

Shipmentandstorage

Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert.

Expirationdate

12months.