MTSCellProliferationColorimetricAssayKit-2500reactions
KitSummary:
•Detectionmethod-Absorbance(490-500nm).
•Applications-Measurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogensandnutrients.
-Analysisofcytotoxicandcytostaticcompoundssuchasanticancerdrugs,toxicagentsandotherpharmaceuticals.
-Assessmentofphysiologicalmediatorsthatinhibitcellgrowth.
SampleType:
•AdherentandsUSPensioncells.
Features&Benefits:
•Simpleone-stepprocedure;takes~4hours.
•High-throughput.
•Theentireassaycanbeperformeddirectlyina96-wellplateanddoesnotrequirewashing/harvesting/orsolubilizationsteps.
Kitcomponents:
•MTSReagent(inelectrocouplingsolution).
Description:
MTSCellProliferationAssayKitisacolorimetricmethodforsensitivequantificationofviablecellsinproliferationandcytotoxicityassay.ThemethodisbasedonthereductionofMTStetrazoliumcompoundbyviablecellstogenerateacoloredformazanproductthatissolubleincellculturemedia.ThisconversionisthoughttobecarriedoutbyNAD(P)H-dependentdehydrogenaseenzymesinmetabolicallyactivecells.Theformazandyeproducedbyviablecellscanbequantifiedbymeasuringtheabsorbanceat490-500nm.Theassaycanbeusedforthemeasurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogens,andnutrients,etc.Itcanalsobeusedfortheanalysisofcytotoxiccompoundslikeanticancerdrugsandmanyothertoxicagentsandpharmaceuticalcompounds.MTSassayisperformedbyaddingthereagentdirectlyintothecellculturemediawithouttheintermittentsteps,whicharerequiredintheroutineMTTassay.Inaddition,thishigh-throughputassayrequiresnowashingorsolubilizationstepandcanbeperformedin96-wellmicrotiterplate.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
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HansaBioMed Life Sciences提供了从人或动物生物流体,细胞条件培养基和植物提取物中纯化大型(> 150 nm)和小型EV(30-150 nm)的专业知识。可以按照我们的标准方案(结合切向流过滤(TFF)和尺寸排阻色谱(SEC))获得EV净化。或者,可以使用差异超速离心和微滤,化学沉淀或免疫亲和法。
随后,对分离出的电动汽车的总蛋白质含量进行定量,并通过Zetaview(Particle Metrix)进行的纳米颗粒跟踪分析(NTA)进行验证。可根据要求通过ELISA,FACS,TEM,Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。