CustommadeExoTESTReadytoUseKit:Personalizedyourresearch.
HansABIoMedprovidestheflexibilitytocreatinganddesigningyourownkitbychoosingamongawidevarietyofreagentsavailableinourshop.
Startbyselectingtheplatethatprovidesthespecificcaptureandenrichmentofexosomesubpopulationofinterest.Pleaserefertothedetailsregardingimmunoplatesasaguide(startingpoint)intheImmunocaptureandIsolationToolssection(seeimmunoplates).
Selecttheexosomestandard.PleaserefertodetailsregardingExosomeStandards(seeexosomestandards).
Selecttheprimaryantibody.PleaserefertotheExosomeBindingAntibodiessection.
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Ifyouhaveadditionalquestions,pleasecontactourtechnicalsupportteamatinfo@exotest.eu.
Application:
ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.
Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.
SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.
Relatedproducts :
Exosomestandards
RNAextractionkit
Immunocaptureandisolationtools
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HansaBioMed Life Sciences提供了从人或动物生物流体,细胞条件培养基和植物提取物中纯化大型(> 150 nm)和小型EV(30-150 nm)的专业知识。可以按照我们的标准方案(结合切向流过滤(TFF)和尺寸排阻色谱(SEC))获得EV净化。或者,可以使用差异超速离心和微滤,化学沉淀或免疫亲和法。
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