ImmunocaptureofoverallexosomesfromhumanbiofluidsandcellmediaandextractionofvesicleassociatedRNAs(20reactions).
Sampletype: | Plasma,serum,urine,cellculturemedia |
Startingmaterial: | Startingfrom0.1mLofBIOLOGicalsample(plasma)perreaction. Wholeplasmaandserumcanbedirectlyusedforcapture. Concentratedurineandcellculturesupernatantsamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Specificity: | ThiskitprovidesexcellentspecificityandhighbindingcapacityforoverallexosomecaptureandRNApurification.HighyieldandqualityofRNAextractedallowsmultipledownstreamanalyses. |
ProductOverview: | ExosomecaptureoccursonImmunobeadsprecoatedwithexosomeassociatedantibodiesenablingforoverallexosomeisolation. Exosomestandardsforpositivecontrolareincludedinthekit. ThekitcontainsallthereagentsnecessaryforRNApurification,includingRNAbindingcolumnsfortotalRNAcapture. |
Exosomestandard | ChosenbytheuserinHBMLyophilizedExosomeStandardslist(seedetails). |
Storageandshipment: | Allthereagentareshippedat4°Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate: | 8months. |
Applications: | DirectcaptureandexosomeRNAextractionfromhumanbiofluidsandcellculturemediawithoutinitialexosomepurificationstep. SimultaneousmiRNAandmRNAprofiling(qRT-PCR,RT-PCR,microarray). |
Relatedproducts :
Exosomestandards
Immunocaptureandisolationtools
Quantificationkits
HansaBioMed净化大型和小型电动汽车
HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。
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