EXO-DNAc-UC:IsolationofcirculatingandEV-associatedDNAfromurineandcellculturemedia.
Sampletype | Cellculturemediaandurine |
Startingmaterial | Startingfrom1mlofcellmediaperreaction.Startingfrom5mlofurine. |
Specifications | ThiskitprovideshighcapacityforpurificationofcirculatingandEVassociatedDNA.PelletafterisolationofEVsandcirculatingDNAcanbetreatedwithDnasetoeliminatecellfreecirculatingDNA.AfterDnasetreatmentDNAextractionfromEVsproceedsfollowingtheidenticalprotocol.EXO-DNAcprovidesanappropriatedDNAconcentratorforconcentratingtheyieldandincreasingthepurityoftheDNAtothelevelsrequiredfordigitalPCRanalysis. |
Productoverview | EVsareisolatedquicklywithDNA-Prepreagent. Exosomestandardsforpositivecontrolareincludedinthekit(1vial,100ug). ThekitcontainsallthereagentsnecessaryforDNApurification,includingDNApurificationcolumnsandDNAconcentratorcolumns.Dnaseisnotprovided. |
Exosomestandard | ChosenbytheuserinHBMLyophilizedExosomeStandardslist(seedetails). |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductdatasheet. |
Expirationdate | 12months. |
Applications | DirectEVscaptureandDNApurificationfromplasmaandserumwithouttimeconsumingEVpurificationsteps. IsolationofgenomicEV-associatedDNAbyDnasetreatment. Profilingofcirculatingand/orEV-associatedgenomicDNA. DetectionofactionablemutationsbydigitalPCRanalysis. IsolatedDNAissuitableforNGS. |
Relatedproducts :
Exosomestandards
RNAextractionkit
Exosomeisolationtools
HansaBioMed净化大型和小型电动汽车
HansaBioMed Life Sciences提供了从人或动物生物流体,细胞条件培养基和植物提取物中纯化大型(> 150 nm)和小型EV(30-150 nm)的专业知识。可以按照我们的标准方案(结合切向流过滤(TFF)和尺寸排阻色谱(SEC))获得EV净化。或者,可以使用差异超速离心和微滤,化学沉淀或免疫亲和法。
随后,对分离出的电动汽车的总蛋白质含量进行定量,并通过Zetaview(Particle Metrix)进行的纳米颗粒跟踪分析(NTA)进行验证。可根据要求通过ELISA,FACS,TEM,Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。