SenescenceDetectionKit-250reactions

KitSummary:

•Sampletype-Cellculturecells,tissues.

•Speciesreactivity-Mammalian.

•Kitsize-250stainings.

•Applications-TheSenescenceDetectionKitisdesignedtohistochemicallydetectspecificsenescenceMarkerindistinctpHinculturedcellsandtissuesections.

Features&Benefits:

•Simpleprocedure;takesaroundthan30minutes.

•Fastandconvenient.

•Thespecifichistochemicalmarkerisonlypresentinsenescentcellsandisnotfoundinpresenescent,quiescentorimmortalcells.

Kitcomponents:

•FixativeSolution(1X).

•X-Gal(150mg,lyophilized).

•StainingSolution(1X).

•StainingSupplement(100X).

Description:

Senescenceisthoughttobeatumorsuppressivemechanismandanunderlyingcauseofaging.Senescencerepresentsanarrestedstateinwhichthecellsremainviable,butnotstimulatedtodividebyserumorpassageinculture.Senescentcellsdisplayincreaseofcellsize,senescence-associatedexpressionofβ-galactosidase(SA-β-Gal)activity,andalteredpatternsofgeneexpression.TheSenescencedetectionkitisdesignedtohistochemicallydetectSA-β-Galactivityinculturedcellsandtissuesections,aknowncharacteristicofsenescentcells.TheSA-β-Galispresentonlyinsenescentcellsandisnotfoundinpresenescent,quiescentorimmortalcells.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。