ProteasomeActivityAssayKit-100reactions

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em350/440nm).

•Sampletype-Wholecelllysates,nuclearandcytoplasmiclysates.

•Speciesreactivity-Mammalian.

•Applications-Specificallymeasuresproteasomeactivityfromotherproteaseactivitypresentinsamples.

Features&Benefits:

•Simpleone-stepprocedure;takesonly30-60minutes.

•Accurateandeasytouse.

Kitcomponents:

•ProteasomeAssayBuffer.

•ProteasomeSubstrate(InDMSO).

•ProteasomeInhibitor(InDMSO).

•AMCStandard(1mMinDMSO).

•PositiveControl.

Description:

Proteasomesareverylarge(20S,26S)proteinassembliesfoundinboththenucleusandcytoplasmofalleucaryotes(andinsomeprocaryotes).TheyareresponsIBLeforthedegradationandrecyclingofproteinswhichhavebeenpreviouslytaggedwithubiquitin.Suchtaggedproteinsaredegradedintopeptidesapproximately7-8aminoacidslongwhicharesubsequentlyfurtherdegraded.The20Sassemblyisthefunctionalproteasestructurewithchymotrypsin-like,trypsin-likeandcaspase-likeproteaseactivities.ProteasomeActivityAssaytakesadvantageofthechymotrypsin-likeactivity,utilizinganAMC-taggedpeptidesubstratewhichreleasesfree,highlyfluorescentAMCinthepresenceofproteolyticactivity.Thekitalsoincludesapositivecontrol(JurkatCelllysatewithsignificantproteasomeactivity)andaspecificproteasomeinhibitorMG-132whichsuppressesallproteolyticactivityduetoproteasomes.Thispermitsdifferentiationofproteasomeactivityfromotherproteaseactivitywhichmaybepresentinsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。