Myeloperoxidase(MPO)ColorimetricActivityAssayKit.

SensitiveAssay,HTS

KitSummary:

•Detectionmethod-Absorbance(412nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluidsetc.

•Speciesreactivity-Mammalian.

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•SensitiveassaysformeasuringMPOaslowas0.05mUperwell.

KitComponents:

•MPOAssayBuffer

•DTNBProbe

•TCEP

•MPOSubstrate

•StopMix

•MPOPositiveControl

Description:

Myeloperoxidase(MPO)isaperoxidaseenzyme(EC1.11.1.7)mostabundantlyexpressedinneutrophilgranulocytes.Itisalysosomalproteinstoredintheazurophilicgranulesoftheneutrophil.MPOcontainsahemepigmentwhichcausesitsgreencolorinsecretionsrichinneutrophils,suchaspusandsomeformsofmucus.MPOcatalyzestheproductionofhypochlorousacid(HClO)fromhydrogenperoxide(H₂O₂)andchlorideanion(Cl-,ortheequivalentfromanon-chlorinehalide).MPOalsooxidizestyrosinetoatyrosylrADIcalusinghydrogenperoxideasanoxidizingagent.InHansABIoMed"sMPOAssayKit,theHClOproducedfromH₂O₂andCl-isreactedwithtaurinetogeneratethetaurinechloramine,whichsubsequentlyreactswiththeTNB2-probetoeliminatecolor(λ=412nm).Thekitprovidesarapid,simple,sensitive,andreliabletestsuitableforhighthroughputactivityassayofMPO.ThiskitcanbeusedtodetectMPOaslowas0.05mUperwell.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。