UricAcidColorimetric/FluorometricAssayKit.
Sensitive,Colorimetric&FluorometricAssay.
KitSummary:
•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)
•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids
•Speciesreactivity-Mammalian.
Features&Benefits:
•Simpleprocedure;takes~40minutes
•Fastandconvenient
•KitcontainsthenecessaryreagentsforaccuratemeasurementofUricacidinbiologicalsamples.
KitComponents:
•UricAcidAssayBuffer
•UricAcidProbe(inDMSO,anhydrous)
•UricAcidEnzymeMix
•UricAcidStandard(2nmol/µl)
Description:
Uricacidinserumistheendproductofpurinemetabolism,andisclearedthroughthekidneybyglomerularfiltration.However,humanoftenlacksthenecessaryenzymecalledurateoxidase(Uricase),andthereforeabnormaluricacidmaybeaccumulatedinblood.Recentevidencesshowthecloseassociationbetweenserumuratelevelandcardiovascularmorbidityandmortality,especiallyamongpersonsathighcardiovascularrisk,includingthosewithhypertension,diabetesandcongestionheartfailure.TheUricAcidAssayKitprovidesaconvenientmeansfordetectinguricacidinbiologicalsamplessuchasserumandurine.Pretreatmentofsamplesarenotrequired.Uricacidlevelcanbemeasuredusingfluorometric(atEx/Em=535/587nm)orcolorimetric(atλ=570nm)methods.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
HansaBioMed净化大型和小型电动汽车
HansaBioMed Life Sciences提供了从人或动物生物流体,细胞条件培养基和植物提取物中纯化大型(> 150 nm)和小型EV(30-150 nm)的专业知识。可以按照我们的标准方案(结合切向流过滤(TFF)和尺寸排阻色谱(SEC))获得EV净化。或者,可以使用差异超速离心和微滤,化学沉淀或免疫亲和法。
随后,对分离出的电动汽车的总蛋白质含量进行定量,并通过Zetaview(Particle Metrix)进行的纳米颗粒跟踪分析(NTA)进行验证。可根据要求通过ELISA,FACS,TEM,Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。