AscorbicAcidColorimetric/FluorometricAssayKit.

Sensitive,Colorimetric&FluorometricAssay

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm),

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids,foodsamples,growthmedium,etc.

•Speciesreactivity-Mammalian,

•Applications-Theassaycandetect0.01-10nmolofascorbicacidperassayinvarioussamples.

Features&Benefits:

•Simpleprocedure;takes~5minutesforthereactiontocomplete

•Fastandconvenient.

KitComponents:

•AscorbicAcidAssayBuffer

•AscorbicAcidProbe(lyophilized)

•Dimethylsulfoxide(DMSO,Anhydrous)

•Catalyst

•AscorbicAcidEnzymeMix(lyophilized)

•AscorbicAcidStandard(20µmole).

Description:

AscorbicAcid(VitaminC)playsanimportantroleinmanybiologicalprocesses.Itisapotentanti-oxidant,anti-inflammatory,anti-viralagent,andanimmunestimulantandispresentisawidevarietyoffoodsandbiologicalspecimens.Itisimportanttobeabletomonitorascorbicacidcontentinthesedifferentsamples.AscorbicAcidAssayKitprovidesarapid,simple,andsensitivemeansofdetectingascorbicacidinvariousbiologicalsamples.Inthisassay,ourproprietarycatalystoxidizesascorbicacidtoproduceaproductthatinteractswiththeascorbicacidprobe,generatingcolorandfluorescence.Ascorbicacidcanbeeasilydeterminedbyeithercolorimetric(spectrophotometryatλ=570nm)orfluorometric(Ex/Em=535/587nm)methods.Theassaycandetect0.01-10nmolofascorbicacidperassayinvarioussamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。