CatalaseActivityColorimetric/FluorometricAssayKit.

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids,growthmedium,foodetc.

•Speciesreactivity-Mammalian

•Applications-Thekitdetectshighpico-unitofcatalaseinsamples.

Features&Benefits:

•Simpleprocedure;takes~30-40minutes

•Fastandconvenient

•SensitiveassaysformeasuringCatalaseinvariousbiologicalsamples

KitComponents:

•CatalaseAssayBuffer

•OxiRed™Probe(inDMSO)

•HRP(lyophilized)

•H₂O₂(3%;0.88M)

•StopSolution

•CatalasePositiveControl(lyophilized)

Description:

Catalase(EC1.11.1.6)isaubiquitousantioxidantenzymethatispresentinnearlyalllivingorganisms.Itfunctionstocatalyzethedecompositionofhydrogenperoxide(H₂O₂)towaterandoxygen.CatalaseAssayKitprovidesahighlysensitive,simple,directandHTS-readyassayformeasuringCatalaseactivityinbiologicalsamples.Intheassay,catalasefirstreactswithH₂O₂toproducewaterandoxygen,theunconvertedH₂O₂reactswithOxiRed™probetoproduceaproduct,whichcanbemeasuredat570nm(Colorimetricmethod)oratEx/Em=535/587nm(fluorometricmethod).Catalaseactivityisreverselyproportionaltothesignal.Thekitdetectshighpico-unitofcatalaseinsamples.

StorageConditions:+4°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。