CytochromecApoptosisAssayKit.

KitSummary:

•Detectionmethod-Westernblotting

•Sampletype-CellsandTissues

•Speciesreactivity-Mammalian

•Applications-Effectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesaneasy-to-useprocedureandreagentsforseparatingamitochondria-enrichedfractionfromcytosol.

Features&Benefits:

•Simpleprocedure;takes~3hours

•Fastandconvenient

•CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.Theprocedureissimpleandstraightforward.Noultracentrifugationisrequired.

KitComponents:

•MitochondriaExtractionBuffer

•5XCytosolExtractionBuffer

•DTT(1M)

•500XProteaseInhibitorCocktail

•Anti-CytochromecmousemAb(0.2mg/ml).

Description:

Cytochromecplaysanimportantroleinapoptosis.Theproteinislocatedinthespacebetweentheinnerandoutermitochondrialmembranes.AnapoptoticstimulustriggersthereleaseofcytochromecfromthemitochondriaintocytosolwhereitbindstoApaf-1.Thecytochromec/Apaf-1complexactivatescaspase-9,whichthenactivatescaspase-3andotherdownstreamcaspases.CytochromecReleasingApoptosisAssayKitprovidesaneffectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesuniqueformulationsofreagentstoisolateahighlyenrichedmitochondriafractionfromcytosol.Theprocedureissosimpleandeasytoperform,noultracentrifugationisrequiredandnotoxicchemicalsareinvolved.CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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