DNADamageQuantificationColorimetricKit.

KitSummary:

•Detectionmethod-Absorbance(450and650nm)

•Speciesreactivity-Mammalian

•Applications-KitprovidesallnecessaryreagentsforconvenientdeterminationofDNAdamage(abasicsites)insampleDNA.

SampleType:

PurifieddamagedDNA

Features&Benefits:

Kitprovidesallnecessaryreagentsfor96-wellplateformat.

KitComponents:

•ARPSolution

•TEBuffer

•GlycogenSolution

•0ARP-DNAStandard

•40ARP-DNAStandard

•DNABindingSolution

•HRP-Streptavidin

•10XWashBuffer

•HRPDeveloper

•96-wellMicroplate(8x12strips).

Description:

Apurinic/apyrimidinic(AP)sitesareoneofthemajortypesofDNAlesionsformedduringthecourseofbaseexcisionandrepairofoxidized,deaminatedoralkylatedbases.Ithasbeenestimatedthatabout2x105baselesionsaregeneratedpercellperday.ThelevelofAPsitesincellscanbeagoodindicatorofDNAlesionandrepairagainstchemicaldamageandcellaging.TheDNADamageQuantificationKitutilizestheARP(AldehydeReactiveProbe)reagentthatreactsspecificallywithanaldehydegroupwhichistheopenringformoftheAPsites.AftertreatingDNAcontainingAPsiteswithARPreagents,APsitesaretaggedwithbiotinresidues,whichcanbequantifiedusingavidin-biotinassayfollowedbyacolorimetricdetection.ThekitprovidesthenecessaryreagentsforconvenientdeterminationofabasicsitesinpurifiedDNAsamplein96-wellplateformat.

StorageConditions:+4°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。