GAPDHActivityAssayKit.

KitSummary:

•Detectionmethod-Absorbance(450nm)

•Applications-Measurementofglyceraldehyde-3-phosphatedehydrogenase(GAPDH)activityinvarioustissues/cells

-Analysisofglycolysisandpentosephosphatepathways.

SampleType:

•Animaltissues:Liver,Heartetc.

•Cellculture:AdherentorsUSPensioncells.

Features&Benefits:

•Simple,rapid&convenient

•CanmeasureGAPDHactivityaslowas100µU/mLinavarietyofsamples.

KitComponents:

•GAPDHAssayBuffer

•GAPDHSubstrate

•GAPDHDeveloper

•NADHStandard(500nmol)

•GAPDHPositiveControl

Description:

GAPDH(Glyceraldehyde-3-PhosphateDehydrogenase;EC1.2.1.12)catalyzestheconversionofGlyceraldehyde-3-Phosphate(GAP)to1,3-BisphosphateGlycerate(BPG)andplaysakeyroleinglycolysis.Theenzymeisinvolvedincellularprocessessuchasapoptosis,membranetrafficking,ironmetabolismandnucleartranslocation.GAPDH(housekeepinggene)expressionisstableandconstitutive.DeregulationofGAPDHactivityisassociatedwithabnormalcellproliferationandcarcinogenesis.AccuratequantitationofGAPDHactivityisimportantfordiagnosingdiseasesandstudyingnormalcellularphysiology.GAPDHActivityAssayKitprovidesasimpleandsensitivemethodformonitoringGAPDHactivityinvarioussamples.Inthisassay,GAPDHcatalyzesconversionofGAPintoBPGandanintermediate,whichreactswithadevelopertoformacoloredproductthatabsorbsmaximallyat450nm.Ourhigh-throughputadaptableassaycandetectGAPDHactivityaslowas100µU/mLinavarietyofsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。