Hydrogen Peroxide Assay Kit.

Sensitive Assay, HTS

Kit Summary:

• Detection method- Absorbance (570 nm)and fluorescence (Ex/Em 535/587 nm)

• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

• Species reactivity- Mammalian

• Applications- The kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring H₂O₂ in biological samples.

Features & Benefits:

• Simple procedure; takes less than ~1 hours

• Fast and convenient

• The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.

Kit Components:

• H₂O₂ Assay Buffer

• OxiRed™ Probe (in anhydrous DMSO)

• HRP

• H₂O₂ Standard (0.88M)

Description:

Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. The Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H₂O₂ in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H₂O₂ to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.

Storage Conditions: +4°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.

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