LipidPeroxidation(MDA)Colorimetric/FluorometricAssayKit.

Sensitive,Colorimetric&FluorometricAssay

KitSummary:

•Detectionmethod-Absorbance(532nm)orFluorescence(Ex/Em532/553nm)

•Sampletype-CellandTissueculturesupernatants,plasmaandotherBIOLOGicalfluids(optimizedbyenduser).

•Speciesreactivity-All

•Applications-ThekitdetectsMDAlevelsaslowas1nmol/wellcolorimetricallyand0.1nmol/wellfluorometrically.

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•Sensitiveassayformeasuringlipidperoxidation(LPA)inawiderangeofsamples.

KitComponents:

•MDALysisBuffer

•PhosphotungsticAcidSolution

•BHT(100X)

•TBA

•MDAStandard(4.17M)

Description:

Quantificationoflipidperoxidationisessentialtoassessoxidativestressinpathophysiologicalprocesses.LipidperoxidationformsMalondialdehyde(MDA)and4-hydroxynonenal(4-HNE),asnaturalbi-products.Measuringtheendproductsoflipidperoxidationisoneofthemostwidelyacceptedassaysforoxidativedamage.HansABIoMedLipidPeroxidationAssayKitprovidesaconvenienttoolforsensitivedetectionoftheMDAinavarietyofsamples.TheMDAinthesampleisreactedwithThiobarbituricAcid(TBA)togeneratetheMDA-TBAadduct.TheMDA-TBAadductcanbeeasilyquantifiedcolorimetrically(λ=532nm)orfluorometrically(Ex/Em=532/553nm).ThisassaydetectsMDAlevelsaslowas1nmol/wellcolorimetricallyand0.1nmol/wellfluorometrically.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

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