GlutathioneReductaseActivityColorimetricAssayKit.

HighlySensitiveAssay,HTS

KitSummary:

•Detectionmethod-Absorbance(405nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,erythrocytelysates,aswellasmanyotherBIOLOGicalfluidsetc.

•Speciesreactivity-Mammalian

•Application-Theassaycandetect0.1–40mU/mlGRinvarioussamples.

Features&Benefits:

•Simpleprocedure;takes~30-40minutes

•Fastandconvenient

KitComponents:

•GRAssayBuffer

•3%H₂O₂

•Catalase

•TNBStandard(5mM)

•DTNB

•NADPH-GNERAT™

•GSSG

•GRPositiveControl(10mU)

Description:

GlutathioneReductase(GR,EC1.8.1.7)catalyzestheNADPH-dependentreductionofoxidizedglutathione(GSSG)toreducedglutathione(GSH),whichplaysanimportantroleintheGSHredoxcyclethatmaintainsadequatelevelsofreducedGSH.AhighGSH/GSSGratioisessentialforprotectionagainstoxidativestress.HansABIoMed’sGlutathioneReductaseAssayKitisahighlysensitive,simple,directandHTS-readycolorimetricassayformeasuringGRactivityinbiologicalsamples.Intheassay,GRreducesGSSGtoGSH,whichreactswith5,5′-Dithiobis(2-nitrobenzoicacid)(DTNB)togenerateTNB2-(yellowcolor,λmax=405nm).Theassaycandetect0.1–40mU/mlGRinvarioussamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

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HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。