BranchedChainAminoAcid(Leu/Ile/Val)ColorimetricAssayKit.

Sensitive,ColorimetricAssay

KitSummary:

•Detectionmethod-Absorbance(450nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-HansABIoMed’sBCAAkitmeasuresBCAA’sintherangeof0to10nmolpersamplewithadetectionlimitof~0.2nmol(~10µMBCAAinsample).

Features&Benefits:

•Simpleprocedure;takeslessthan~40minutes

•Fastandconvenient

KitComponents:

•BCAAAssayBuffer

•BCAAEnzymeMix

•WSTSubstrateMix

•LeuStandard(1µmol)

Description:

Thebranched-chainaminoacidsorBCAA’s,refertotheaminoacidswithnon-linearaliphaticside-chains,namelyleucine,isoleucineandvaline.Thesethreeessentialaminoacidsmakeupapproximately1/3ofskeletalmuscleinthehumanbody.BCAA’sarecurrentlyusedclinicallytoaidintherecoveryofburnvictims,aswellasforstrengthsupplementationforathletes.BCAA’s,primarilyLeu,canstimulateinsulinsecretion.TheBCAA’shavealsobeenimplicatedinawiderangeofotherphysiologicaleffects.HansaBioMed"sBCAAAssayKitprovidesasimpleconvenientmeansofmeasuringtheBCAA’sinavarietyofbiologicalsamples.ThekitutilizesanenzymeassayinwhichBCAAisoxidativelydeaminated,producingNADHwhichreducestheprobe,generatingacoloredproduct(λmax=450nm).TheBCAAkitmeasuresBCAA’sintherangeof0to10nmolpersamplewithadetectionlimitof~0.2nmol(~10µMBCAAinsample).BCAA’sarepresentinserum~0.1-0.4mMeach(~0.125-1.5mMcombined).

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed净化大型和小型电动汽车

HansaBioMed Life Sciences提供了从人或动物生物流体，细胞条件培养基和植物提取物中纯化大型（> 150 nm）和小型EV（30-150 nm）的专业知识。可以按照我们的标准方案（结合切向流过滤（TFF）和尺寸排阻色谱（SEC））获得EV净化。或者，可以使用差异超速离心和微滤，化学沉淀或免疫亲和法。

随后，对分离出的电动汽车的总蛋白质含量进行定量，并通过Zetaview（Particle Metrix）进行的纳米颗粒跟踪分析（NTA）进行验证。可根据要求通过ELISA，FACS，TEM，Western印迹进行其他表征。纯化后的电动汽车可以根据需要冻干或冷冻运送给客户。