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立陶宛BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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Divbio Science Europe
多元化生物技术 bvP.O. Box 924850 AB Ulvenhout荷兰
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列支敦士登BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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拉脱维亚BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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Divbio Science Europe
多元化生物技术 bvP.O. Box 924850 AB Ulvenhout荷兰
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Newmarket Scientific9-10 Rosemary HouseLanwades Business ParkKennett, NewmarketCB8 7PNUnited Kingdom
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冰岛BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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塞浦路斯BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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克罗地亚BIA d.o.o.Cesta v Gorice 34SI-1000 LjubljanaSlovenia
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Gentaur 保加利亚 EoodUl。 Iskar 531191 Kokalyane, Sofia, Bulgaria
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BIOZOL Diagnostica Vertrieb GmbH Leipziger Straße 485386 Eching, 德国
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波斯尼亚和黑塞哥维那BIA d.o.o.Cesta v Gorice 34SI-1000 LjubljanaSlovenia
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Capsugel Australia Pty Ltd.Suite 610, 12 Century Circuit Norwest, NSW 2153Australia
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EV 标记物的蛋白质组学和转录组学分析：
质谱分析：样品制备和 Q Exactive Plus 2h nano-LC/MS/MS 分析，以及基于 MaxQuant 的识别和定量。RNA seq ：使用 Nextflex 试剂盒制备文库。通过 Illumina Nextseq 500 完成测序服务定制产品我们可以通过在我们目录中的各种试剂和工具中进行选择来设计和创建您的个性化试剂盒。![\"\"](\"http://www.hansabiomed.eu/images/exofacs1.png\")
Proteasome Activity Assay Kit - 100 reactions
Kit Summary:
• Detection method - Fluorescence (Ex/Em 350/440nm).
• Sample type - Whole cell lysates, nuclear and cytoplasmic lysates.
• Species reactivity - Mammalian.
• Applications - Specifically measures proteasome activity from other protease activity present in samples.
Features & Benefits:
• Simple one-step procedure; takes only 30-60 minutes.
• Accurate and easy to use.
Kit components:
• Proteasome Assay Buffer.
• Proteasome Substrate (In DMSO).
• Proteasome Inhibitor (In DMSO).
• AMC Standard (1 mM in DMSO).
• Positive Control.
Description:
Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes). They are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
Storage Conditions: -20°C.
Shipping Conditions: gel pack.
USAGE: For Research Use Only! Not For Use in Humans.
